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ab185033 goat polyclonal igg anti gfra2 r d systems  (R&D Systems)


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    R&D Systems ab185033 goat polyclonal igg anti gfra2 r d systems
    Ab185033 Goat Polyclonal Igg Anti Gfra2 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+gdnf+polyclonal+antibody/Human%2FMouse+GFR+alpha-2%2FGDNF+R+alpha-2+Antibody/pmc12533021__41419_2025_8056_MOESM11_ESM-2-138-143
    Average 94 stars, based on 37 article reviews
    ab185033 goat polyclonal igg anti gfra2 r d systems - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: Long-term controlled GDNF over-expression reduces dopamine transporter activity without affecting tyrosine hydroxylase expression in the rat mesostriatal system.
    Article Snippet: .. Floating sections were immersed for 30 min in 3% H2O2 to inactivate endogenous peroxidase, and incubated for 60min at RT in 4% normal donkey serum (NDS, Jackson ImmunoResearch,West Grove, PA) in PBS, containing 0.05% Triton X-100 (TX-100, Sigma), and overnight in PBS containing 2% NDS and a goat anti-GDNF polyclonal antibody (1:500; R&D Systems, Minneapolis, MN). .. After several rinses, sections were incubated for 2 h in biotinylated donkey anti-goat antiserum (1:1000, Jackson ImmunoResearch) and 1:200 NDS in PBS.

    Article Title: Brain-Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor Are Required Simultaneously for Survival of Dopaminergic Primary Sensory Neurons In Vivo
    Article Snippet: .. To detect endogenous GDNF, sections containing the carotid body were hydrated in 0.1 m PBS, quenched with 0.5% H 2 0 2 in methanol for 15 min, rinsed in PBS, soaked for 1 hr in dilution buffer (PBS containing 10% donkey serum and 0.3% Triton X-100), blocked with avidin (15 min; Vector Laboratories, Burlingame, CA), rinsed with PBS, blocked with biotin (15 min; Vector Laboratories), rinsed again with PBS, placed in dilution buffer (15 min), then incubated overnight at room temperature with a goat anti-GDNF polyclonal antibody (catalog #AF-212-NA; 0.125 μg/ml diluted in 0.1 m PBS with 10% donkey serum; R & D Systems, Minneapolis, MN). .. Sections were then rinsed thoroughly with PBS, incubated for 30 min with biotinylated donkey anti-goat IgG (catalog #705-066-147, diluted 1:1000 in 0.1 m PBS with 10% donkey serum; Jackson ImmunoResearch, West Grove, PA), rinsed repeatedly with PBS, reacted with ABC Elite reagent (30 min, 1:100 in 0.5 m NaCl-PBS; Vector Laboratories), rinsed in NaCl-PBS (10 min) followed by TNT (0.1 m Tris-HCl, 0.15 m NaCl, and 0.5% Tween 20; 2 × 10 min), and TNB (0.1 m Tris-HCl, 0.15 m NaCl, and 0.5% blocking reagent from TSA kit, 10 min; NEN Life Science Products, Boston, MA), then incubated in biotinyl tyramide solution (5 min; 1:50; TSA kit).

    Article Title: Brain-Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor Are Required Simultaneously for Survival of Dopaminergic Primary Sensory NeuronsIn Vivo
    Article Snippet: .. To detect endogenous GDNF, sections containing the carotid body were hydrated in 0.1 M PBS, quenched with 0.5% H202 in methanol for 15 min, rinsed in PBS, soaked for 1 hr in dilution buffer (PBS containing 10% donkey serum and 0.3% Triton X-100), blocked with avidin (15 min; Vector Laboratories, Burlingame, CA), rinsed with PBS, blocked with biotin (15 min; Vector Laboratories), rinsed again with PBS, placed in dilution buffer (15 min), then incubated overnight at room temperature with a goat anti-GDNF polyclonal antibody (catalog #AF-212-NA; 0.125 mg/ml diluted in 0.1 M PBS with 10% donkey serum; R & D Systems, Minneapolis, MN). .. Sections were then rinsed thoroughly with PBS, incubated for 30 min with biotinylated donkey anti-goat IgG (catalog #705-066-147, diluted 1:1000 in 0.1 M PBS with 10% donkey serum; Jackson ImmunoResearch, West Grove, PA), rinsed repeatedly with PBS, reacted with ABC Elite reagent (30 min, 1:100 in 0.5 M NaCl-PBS; Vector Laboratories), rinsed in NaCl-PBS (10 min) followed by TNT (0.1 M Tris-HCl, 0.15 M NaCl, and 0.5% Tween 20; 2 3 10 min), and TNB (0.1 M Tris-HCl, 0.15 M NaCl, and 0.5% blocking reagent from TSA kit, 10 min; NEN Life Science Products, Boston, MA), then incubated in biotinyl tyramide solution (5 min; 1:50; TSA kit).

    Avidin-Biotin Assay:

    Article Title: Brain-Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor Are Required Simultaneously for Survival of Dopaminergic Primary Sensory Neurons In Vivo
    Article Snippet: .. To detect endogenous GDNF, sections containing the carotid body were hydrated in 0.1 m PBS, quenched with 0.5% H 2 0 2 in methanol for 15 min, rinsed in PBS, soaked for 1 hr in dilution buffer (PBS containing 10% donkey serum and 0.3% Triton X-100), blocked with avidin (15 min; Vector Laboratories, Burlingame, CA), rinsed with PBS, blocked with biotin (15 min; Vector Laboratories), rinsed again with PBS, placed in dilution buffer (15 min), then incubated overnight at room temperature with a goat anti-GDNF polyclonal antibody (catalog #AF-212-NA; 0.125 μg/ml diluted in 0.1 m PBS with 10% donkey serum; R & D Systems, Minneapolis, MN). .. Sections were then rinsed thoroughly with PBS, incubated for 30 min with biotinylated donkey anti-goat IgG (catalog #705-066-147, diluted 1:1000 in 0.1 m PBS with 10% donkey serum; Jackson ImmunoResearch, West Grove, PA), rinsed repeatedly with PBS, reacted with ABC Elite reagent (30 min, 1:100 in 0.5 m NaCl-PBS; Vector Laboratories), rinsed in NaCl-PBS (10 min) followed by TNT (0.1 m Tris-HCl, 0.15 m NaCl, and 0.5% Tween 20; 2 × 10 min), and TNB (0.1 m Tris-HCl, 0.15 m NaCl, and 0.5% blocking reagent from TSA kit, 10 min; NEN Life Science Products, Boston, MA), then incubated in biotinyl tyramide solution (5 min; 1:50; TSA kit).

    Article Title: Brain-Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor Are Required Simultaneously for Survival of Dopaminergic Primary Sensory NeuronsIn Vivo
    Article Snippet: .. To detect endogenous GDNF, sections containing the carotid body were hydrated in 0.1 M PBS, quenched with 0.5% H202 in methanol for 15 min, rinsed in PBS, soaked for 1 hr in dilution buffer (PBS containing 10% donkey serum and 0.3% Triton X-100), blocked with avidin (15 min; Vector Laboratories, Burlingame, CA), rinsed with PBS, blocked with biotin (15 min; Vector Laboratories), rinsed again with PBS, placed in dilution buffer (15 min), then incubated overnight at room temperature with a goat anti-GDNF polyclonal antibody (catalog #AF-212-NA; 0.125 mg/ml diluted in 0.1 M PBS with 10% donkey serum; R & D Systems, Minneapolis, MN). .. Sections were then rinsed thoroughly with PBS, incubated for 30 min with biotinylated donkey anti-goat IgG (catalog #705-066-147, diluted 1:1000 in 0.1 M PBS with 10% donkey serum; Jackson ImmunoResearch, West Grove, PA), rinsed repeatedly with PBS, reacted with ABC Elite reagent (30 min, 1:100 in 0.5 M NaCl-PBS; Vector Laboratories), rinsed in NaCl-PBS (10 min) followed by TNT (0.1 M Tris-HCl, 0.15 M NaCl, and 0.5% Tween 20; 2 3 10 min), and TNB (0.1 M Tris-HCl, 0.15 M NaCl, and 0.5% blocking reagent from TSA kit, 10 min; NEN Life Science Products, Boston, MA), then incubated in biotinyl tyramide solution (5 min; 1:50; TSA kit).



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